The overall objective of this WP is the adaptation and validation of multiplex assays for simple and reliable pathogen detection in small field laboratories. Both direct genome and antigen detection methods will be employed. Moreover, methods that allow for basic typing of selected viruses will be tested.
The following diseases have been selected as the most important and reasonable for this working package:
- Cattle cluster “vesicular diseases” simultaneous detection and differentiation of BTV/EHDV/FMDV and BVDV
- Pig cluster respiratory and hemorrhagic diseases simultaneous detection and differentiation of PRRSV, PCV2, ASFV, and CSFV
- Chicken cluster “fowl plague” simultaneous detection and differentiation of AIV and NDV
- Honey bee virus cluster
All disease clusters combine important notifiable, but also endemic diseases, and take especially the importance of differential diagnosis in screening programs and outbreak situations into consideration. For some methodologies, restrictions to certain pathogens will apply.
Based on capacity considerations, the methods will be divided into direct genome detection methods (FLI leader)
and techniques for direct antigen detection (INTA leader)
In detail, the following tasks will be executed:
- Evaluation, adaptation and validation of direct genome detection methods including necessary extraction steps utilizing low density DNA chip systems, and LAMP assays.
- Development and validation of direct antigen detection methods using low density microarrays, digitally-encoded micro-particles, multiplex antigen-ELISA, Q-ELISA, and AlphaLISA technology. Please login or register to see the full page.